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Proceedings Paper

Effects of LLLT on malignant cells: study in vitro
Author(s): Antonio Luiz Barbosa Pinheiro; Silene Carneiro Nascimento; Alessandro L. B. Vieira; Aluizio Barros Rolim; Pedro Soriano da Silva; Aldo Brugnera; Fatima A. A. Zanin
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Paper Abstract

In addition to our previous report on the effects of LLLT on the proliferation of laryngeal carcinoma cells in which it was found that irradiation H.Ep.2 cells with 670nm laser results in increased cell proliferation, ti was decided to evaluate the effect of increased does of laser light. The aim of this study was to assess the effect off 635 and 670 nm laser irradiation of H.Ep.2 cells in vitro using MTT. The cells, obtained from SCC of the larynx, were routinely processed from defrost to the experimental condition. The cultures were kept either at 5 or 10 percent of FBS. Twenty- four hours after transplantation, the cells were irradiated with laser light at local light doses between 0.04 and 4.8 X 104 Jm-2. For 670 nm, significant differences in the proliferation were observed between the two concentrations of FBS and between irradiated cultures and controls. Although the results were not significant, 635 nm irradiated cells also proliferated more than non- irradiated ones. This occurred under both conditions of nutrition. It is concluded, that irradiation with 670 nm laser light applied at doses between 0.04 and 4.8 X 104 Jm-2 could significantly increase proliferation of laryngeal cancer cells.

Paper Details

Date Published: 27 April 2001
PDF: 5 pages
Proc. SPIE 4249, Lasers in Dentistry VII, (27 April 2001); doi: 10.1117/12.424490
Show Author Affiliations
Antonio Luiz Barbosa Pinheiro, Univ. Federal da Bahia and Univ. do Vale do Paraiba (Brazil)
Silene Carneiro Nascimento, Univ. Federal de Pernambuco (Brazil)
Alessandro L. B. Vieira, Dentist (Brazil)
Aluizio Barros Rolim, Dentist (Brazil)
Pedro Soriano da Silva, Univ. Federal de Pernambuco (Brazil)
Aldo Brugnera, Univ. Federal do Rio de Janeiro and Instituto Brugnera (Brazil)
Fatima A. A. Zanin, Univ. Federal do Rio de Janeiro and Instituto Brugnera (Brazil)

Published in SPIE Proceedings Vol. 4249:
Lasers in Dentistry VII
Peter Rechmann; Daniel Fried; Thomas Hennig, Editor(s)

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