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Proceedings Paper

PDT-induced apoptosis: what are the critical molecular targets
Author(s): Nancy L. Oleinick; Irina Belichenko; Song-mao Chiu; Minh C. Lam; Rachel L. Morris; Liang-yan Xue
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Paper Abstract

Early molecular damages have been studied in a series ofhuman tumor and rodent cell lines following photodynamic therapy (PDT) sensitized by the silicon phthalocyanine Pc 4. Pc 4 preferentially localizes in mitochondria, and upon photoirradiation, immediate photodamage to the anti-apoptotic oncoprotein Bcl-2 is observed. The loss ofthe native 26-kDa protein, as evidenced by western blot analysis, is accompanied by the generation of a 23 -kDa fragment from a small portion of the molecules as well as a variety ofhigher molecular weight protein bands indicative ofphotochemical crosslinking ofBcl-2 to itself, to (pro-apoptotic) homologs, or to other nearby proteins. The changes in Bcl-2 are apparent immediately upon exposure ofPc 4-loaded cells to activating red light, occur in the cold, and are not dependent upon caspase-3 or other proteases. Crosslinking is also observed for the intermediate filament protein vimentin. The results implicate Bcl-2 (and perhaps vimentin) as important molecular targets that lead to apoptosis in Pc 4-PDT-treated cells.

Paper Details

Date Published: 9 April 2001
PDF: 6 pages
Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); doi: 10.1117/12.424444
Show Author Affiliations
Nancy L. Oleinick, Case Western Reserve Univ. (United States)
Irina Belichenko, Case Western Reserve Univ. (United States)
Song-mao Chiu, Case Western Reserve Univ. (United States)
Minh C. Lam, Case Western Reserve Univ. (United States)
Rachel L. Morris, Case Western Reserve Univ. (United States)
Liang-yan Xue, Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 4248:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X
Thomas J. Dougherty, Editor(s)

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