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Proceedings Paper

Measurement of intrinsic photobleaching rate by fluorescence microsampling of tissue
Author(s): Brian W. Pogue; Gregory P. Fournier; Altug R. Akay; Gregory C. Burke; P. Jack Hoopes
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Paper Abstract

Recently there is some interest in using photobleaching rates in vivo to correctly interpret dose deposition, and even to predict the dose deposited within tissue. There are many factors causing photobleaching rate changes related to localization, transport and oxygen availability which can confound the interpretation of a bleaching rate measured in vivo, and perhaps the most problematic issue is that the fluorescence signal can come from multiple depths within the tissue and this depth can change during the course of treatment. In this study we examine the use of a micro-fiber bundle designed to sample fluorescence from superficial depths, and use this device to illustrate that spatially localized measurements can be used to extract the intrinsic photobleaching rate of a photosensitizer within a scattering medium. At the same time, while the depth from which the tissue signal originates can be confined, in vivo measurements illustrate that multiple bleaching rates exist in vivo in addition to the spatial problem. This latter observation, suggests that bleaching in tissue is complicated enough, that spatially localized measures of intrinsic fluorescence photobleaching are needed to allow mechanistic interpretation from in vivo measurements.

Paper Details

Date Published: 9 April 2001
PDF: 7 pages
Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); doi: 10.1117/12.424438
Show Author Affiliations
Brian W. Pogue, Dartmouth College (United States)
Gregory P. Fournier, Dartmouth College (United States)
Altug R. Akay, Dartmouth College (United States)
Gregory C. Burke, Aurora Optics, Inc. (United States)
P. Jack Hoopes, Dartmouth College (USA) and Dartmouth Medical School (United States)


Published in SPIE Proceedings Vol. 4248:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X
Thomas J. Dougherty, Editor(s)

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