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Proceedings Paper

Hyperoxygenation enhances photodynamic therapy tumor cure
Author(s): Fred W. Hetzel; Abdus Shakil; Jill Beckers; Qun Chen
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Paper Abstract

Tumor hypoxia, either pre-existing or as a result of oxygen bleaching during PDT light irradiation, can significantly reduce the effectiveness of a PDT treatment. To overcome the effect oftumor hypoxia, we propose using supplemental hyperoxygenation during a PDT treatment. The mechanism for the tumor cure enhancement ofthe combined therapy is investigated in the study by using an in vivo/in vitro assay. Tumors treated with PDT/hyperoxygenation are harvested at various times after the light irradiation and cultured in vitro for colony formation, thus separating the direct cell killing by PDT and secondary vascular effect. The results show that, when combined with hyper-oxygenation, the cell survival (colony formation) rate immediately after a PDT treatment is significantly improved over that treated without hyper-oxygenation, suggesting an enhanced direct cell killing. Cell survival rates were unchanged for the first 4 hours after a PDT treatment, but decreased significantly at 1 8 hours, suggesting that secondary vascular damage did take effect, but not until a period of at least 4 hours after light irradiation. These results further confirm our earlier observation that when a PDT treatment is combined with hyper-oxygenation, it can be more effective in controlling hypoxic tumors.

Paper Details

Date Published: 9 April 2001
PDF: 4 pages
Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); doi: 10.1117/12.424435
Show Author Affiliations
Fred W. Hetzel, HealthONE Alliance (United States)
Abdus Shakil, HealthONE Alliance (United States)
Jill Beckers, HealthONE Alliance (United States)
Qun Chen, HealthONE Alliance (United States)

Published in SPIE Proceedings Vol. 4248:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X
Thomas J. Dougherty, Editor(s)

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