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Proceedings Paper

Excimer laser ablation of plasma polymers for cell and tissue culture applications
Author(s): Helmut Thissen; Jason P. Hayes; Patrick G. Hartley; Graham Johnson; Erol C. Harvey; Hans J. Griesser
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Paper Abstract

The two-dimensional control of cell adhesion is desired for a number of cell- and tissue culture applications. Thus, a suitable method for the two-dimensional control over surface chemistry, which leads to the display of cell-adhesive and non-adhesive signals is required. In our study, allylamine plasma polymer (ALAPP) deposition has been used to provide a cell-adhesive substrate, while additional grafting of poly(ethylene oxide) (PEO) on ALAPP surfaces has been used to prevent cell adhesion. Two-dimensional control over the surface chemistry was achieved using excimer laser ablation. Ablation experiments were carried out using a 248 nm excimer laser with energy densities of 17 - 1181 mJ/cm2 and 1 - 16 pulses per area. Results obtained by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) show controlled thickness ablation of the plasma polymer and the additional PEO graft polymer. Cell culture experiments using bovine corneal epithelial cells show that two-dimensional control of cell adhesion can be achieved by using appropriate masks in the laser beam.

Paper Details

Date Published: 6 April 2001
PDF: 9 pages
Proc. SPIE 4234, Smart Materials, (6 April 2001); doi: 10.1117/12.424405
Show Author Affiliations
Helmut Thissen, CSIRO (Australia)
Jason P. Hayes, Industrial Research Institute Swinburne (Australia)
Patrick G. Hartley, CSIRO (Australia)
Graham Johnson, CSIRO (Australia)
Erol C. Harvey, Industrial Research Institute Swinburne (Australia)
Hans J. Griesser, CSIRO (Australia)

Published in SPIE Proceedings Vol. 4234:
Smart Materials
Alan R. Wilson; Hiroshi Asanuma, Editor(s)

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