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Proceedings Paper

Detection and identification of animal and food pathogens using time-resolved fluorescence
Author(s): Peter E. Andreotti; Richard Meyer; Thomas Campbell; Michael T. Goode; Deborah L. Menking; Emily D. Myers; Tom Palenius; Larry H. Stanker
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Paper Abstract

Time-reso1ved fluorescence immunoassays using dissociation enhanced lanthanide fluorescence have been developed for detection and identification of animal and food pathogens including Clostridium botulinum toxins A and B, Staphylococcus enterotoxm B (SEB), Bacillus anthracis, E. coil 0157:H7, Salmonella, Listeria and Campylobacter. Both double antibody sandwich inimunoassays and microwell filterplate assays have been developed using polyclonal, monoclonal and phage display antibodies labeled with fluorescent europium or samarium lanthanide chelates. Multiplexed dual label eiiropium and samarium assays have been performed for Salmonella simultaneously with E. coli 0157:H7, Salmonella and Listeria. Results from different laboratories are presented to demonstrate that these time-resolved fluorescent immunoassays have high sensitivity and specificity with excellent reproducibility.

Paper Details

Date Published: 13 March 2001
PDF: 10 pages
Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); doi: 10.1117/12.418741
Show Author Affiliations
Peter E. Andreotti, PerkinElmer Inc. (United States)
Richard Meyer, Centers for Disease Control and Prevention (United States)
Thomas Campbell, PerkinElmer Inc. (United States)
Michael T. Goode, U.S. Army Soldier and Biological Chemical Command (United States)
Deborah L. Menking, U.S. Army Soldier and Biological Chemical Command (United States)
Emily D. Myers, U.S. Army Soldier and Biological Chemical Command (United States)
Tom Palenius, PerkinElmer Inc. (Finland)
Larry H. Stanker, U.S. Department of Agriculture (United States)


Published in SPIE Proceedings Vol. 4206:
Photonic Detection and Intervention Technologies for Safe Food
Yud-Ren Chen; Shu-I Tu, Editor(s)

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