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Proceedings Paper

Impedance spectroscopy and biochip sensor for detection of Listeria monocytogenes
Author(s): Arun K. Bhunia; Ziad W. Jaradat; Kristen Naschansky; Mindy Shroyer; Mark T. Morgan; Rafael Gomez; Rashid Bashir; Mike Ladisch
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Paper Abstract

Listeria monocytogenes is a deadly foodbome human pathogen. Its ubiquitous nature and its ability to grow at refrigeration temperatures makes this organism a difficult one to control. High-volume processing of food products and poor sanitary conditions of the processing plants often allow this organism to be present in processed, ready-to-eat (RTE) foods. Improved processing along with real-time detection could reduce the incidence of this pathogen. Conventional methods can detect this pathogen accurately, but take several days (2-7d) to complete, which is not practical considering the short shelf-life and cost of storage of RTE foods. Biosensor based approaches were adopted for sensitive detection of Listeria. Antibody-coupled fiber optic and microelectrical-mechanical system (MEMS) biochips were designed and examined for direct detection of L. monocytogenes from liquid samples. Also, interdigitated microsensor electrode (IME) chip and spectrofluorometer were used to measure L. monocytogenes interaction with mammalian cells (cytopathogenic activities) for indirect detection. Preliminary data generated using laboratory cultures of Listeria species indicated that L. monocytogenes could be detected in 30 mm to 1 h 30 mm depending on the techniques used.

Paper Details

Date Published: 13 March 2001
PDF: 8 pages
Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); doi: 10.1117/12.418740
Show Author Affiliations
Arun K. Bhunia, Purdue Univ. (United States)
Ziad W. Jaradat, Purdue Univ. (United States)
Kristen Naschansky, Purdue Univ. (United States)
Mindy Shroyer, Purdue Univ. (United States)
Mark T. Morgan, Purdue Univ. (United States)
Rafael Gomez, Purdue Univ. (United States)
Rashid Bashir, Purdue Univ. (United States)
Mike Ladisch, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 4206:
Photonic Detection and Intervention Technologies for Safe Food
Yud-Ren Chen; Shu-I Tu, Editor(s)

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