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Proceedings Paper

Control of protein absorption for MEMs and microfluidic applications on silicon surfaces using silane-based self-assembled monolayers of an oligo(ethylene oxide) derivative
Author(s): Thomas W. Schneider; Laura E. Aloi; Robert C. White
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Paper Abstract

Microfabricated devices are becoming increasingly important in biotechnology, especially in the areas of drug delivery, biological fluid analysis, and biological sensors. Interactions between fluid constituents and device surfaces become important when device dimensions reach the micrometer scale. We are reporting the synthesis and application of an octyl trichlorosilane ether of a short chain mono methylated poly(ethylene glycol) (PEG8,7) for control of protein adsorption on silicon surfaces. Self-assembled monolayers (SAMs) of the PEG8,7, a commercially available long chain poly(ethylene glycol) silane (PEG 5000), and 1H,1H,2H,2H,- perfluorooctyltrichlorosilane (13F) were prepared. These three SAM surfaces and uncoated silicon oxide were compared for control of protein adsorption of bovine serum albumin (BSA) under nonflow conditions. PEG8,7 was shown to be the best surface for inhibiting protein adsorption. The surfaces were analyzed using X-ray photoelectron spectroscopy (XPS) to quantify protein adsorption.

Paper Details

Date Published: 26 February 2001
PDF: 7 pages
Proc. SPIE 4205, Advanced Environmental and Chemical Sensing Technology, (26 February 2001); doi: 10.1117/12.417442
Show Author Affiliations
Thomas W. Schneider, Science Applications International Corp. (United States)
Laura E. Aloi, Medtronics, Inc. (United States)
Robert C. White, Science Applications International Corp. (United States)


Published in SPIE Proceedings Vol. 4205:
Advanced Environmental and Chemical Sensing Technology
Tuan Vo-Dinh; Stephanus Buettgenbach, Editor(s)

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