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Proceedings Paper

Computed tomography of the periphery blood lymphocytes and erythrocytes
Author(s): Elena A. Streletskaya; Julia K. Novoderzhkina; Gennady N. Vishnyakov; Gennady G. Levin; Gennady I. Kozinets
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Paper Abstract

Many biological objects, including blood cells, are optically transparent. They don't absorb light and only change optical path length by variations of object's thickness or its refractive index variations. The phase shifting interferometry is used for the visualization of the phase information and the obtaining quantitative properties of cells. Cells are fixed by glutaraldehyde and mounted into immersion liquid for influences of refraction reduction. Blood cell's interferogram contains the information about space distribution refractive index. The phase-shift method was used for its reconstruction. This method includes record of four interferograms used different values of phase-shift of the reference wave. Many blood cells' interferograms are obtained (lymphocytes and erythrocytes). The shape, size and density of cells are measurement. We suppose that these date will be ones of the early diagnostically criteria of hematological diseases. Experimental results for interferometric microscopy and tomography of blood cells are presented.

Paper Details

Date Published: 19 January 2001
PDF: 7 pages
Proc. SPIE 4158, Biomonitoring and Endoscopy Technologies, (19 January 2001); doi: 10.1117/12.413804
Show Author Affiliations
Elena A. Streletskaya, Hematology Scientific Ctr. (Russia)
Julia K. Novoderzhkina, Hematology Scientific Ctr. (Russia)
Gennady N. Vishnyakov, All-Russian Research Institute for Optophysical Measurements (Russia)
Gennady G. Levin, All-Russian Research Institute for Optophysical Measurements (Russia)
Gennady I. Kozinets, Hematology Scientific Ctr. (Russia)

Published in SPIE Proceedings Vol. 4158:
Biomonitoring and Endoscopy Technologies
Israel Gannot; Yuri V. Gulyaev; Theodore G. Papazoglou; Christiaan F. P. van Swol, Editor(s)

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