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Proceedings Paper

Enzyme/indicator optrodes for detection of heavy metal ions and pesticides
Author(s): Alexei V. Nabok; Asim K. Ray; Nickolaj F. Starodub; Kenneth P. Dowker
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Paper Abstract

Composite films containing enzyme and indicator molecules were produced by means of polyelectrolyte self-assembly. These membranes provide two functions: (i) molecular recognition of the substratum by respective enzyme, and (ii) optrode transducing, when the products o the substratum decomposition affect optical spectra of indicator molecules. Apart from direct registration of enzyme reactions, inhibition reactions can also be monitored with this method. Particularly, heavy metal salts and phosphor organic pesticides acting as inhibitors for Urease and Cholinesterase, respectively, were registered. Composite PESA films were deposited onto glass slides and consisted of several layers of poly(alylamine) hydrochloride (PAA) alternated with indicator molecules, either Cyclo-tetra- chromotropylene or Thymol Blue, both containing SO3- Na+ groups. Then a few layers of PAA/enzyme were deposited on top. A typical structure of the samples was (PAA/Indicator)n/(PAA/Enzyme)m/PAA with n equals 1-5. The obtained films were characterized with UV-visible absorption spectroscopy. The effect of the substrate decomposition on the UV-vis spectra of respective indicator molecules was studied. The inhibition of enzymes Urease and Cholinesterase by heavy metal ions and phosphor organic pesticide, respectively was found. The results obtained show the prospects towards development of optical enzyme sensor arrays.

Paper Details

Date Published: 29 December 2000
PDF: 10 pages
Proc. SPIE 4200, Biochemical and Biomolecular Sensing, (29 December 2000); doi: 10.1117/12.411720
Show Author Affiliations
Alexei V. Nabok, Sheffield Hallam Univ. (United Kingdom)
Asim K. Ray, Sheffield Hallam Univ. (United Kingdom)
Nickolaj F. Starodub, A.V. Palladin Institute of Biochemistry (Ukraine)
Kenneth P. Dowker, Sheffield Hallam Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 4200:
Biochemical and Biomolecular Sensing
Robert A. Lieberman, Editor(s)

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