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Proceedings Paper

Response of the fluorescence of tagged proteins on light-assisted modified polymer surfaces
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Paper Abstract

The fluorescence of fluorescently tagged proteins on functionalized polymer surfaces shifts towards higher wavelengths. Two types of polymers for light-assisted surface manipulation have been used, namely diazo-naphto- quionone/novolak (DNQ/N) resist and poly)tert-butyl- methacrylate). The proteins were either hydrophobicity- attached; covalently linked; or specific protein-protein recognition. We observed that on hydrophilic surfaces the fluorescence is shifted towards lower wavelengths. This parasitic effect has to be taken into account when 'reading' biochips but it can be also used for the 'alignment' of the fluorescence of the fluorescently tagged proteins on the same wavelengths via the manipulation of the properties of the substrate polymer.

Paper Details

Date Published: 29 December 2000
PDF: 6 pages
Proc. SPIE 4200, Biochemical and Biomolecular Sensing, (29 December 2000); doi: 10.1117/12.411711
Show Author Affiliations
Dan V. Nicolau, Swinburne Univ. (Australia) and Osaka National Research Institute (Australia)
Takahisa Taguchi, Osaka National Research Institute (Japan)
Hitoshi Suzuki, Communications Research Lab. (Japan)

Published in SPIE Proceedings Vol. 4200:
Biochemical and Biomolecular Sensing
Robert A. Lieberman, Editor(s)

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