Share Email Print
cover

Proceedings Paper

Somatic S-phase pairing of homologous chromosome 3 in interphase nuclei of human peripheral blood lymphocytes
Author(s): Shamci Monajembashi; Alexander Rapp; Michael Hausmann; Heike Dittmar; Karl-Otto Greulich
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Distances of homologous centromeres and telomeres of human chromosomes were interactively measure din relation to the nuclear diameter. In total about 2000 cell nuclei were acquired by fluorescence microscopy. Here the results are presented for two color fluorescence in situ hybridization (FISH) applied to lymphocyte cell nuclei using commercially available DNA probes for chromosome 3 centromere and 3p- telomere. In 89 cell nuclei (66%) of the homologous centromeres had a distance Dc smaller than 15 percent of the nuclear diameter (dn). For these per definition classified 'paired' centromeres an increased frequency of small distances of homologous telomeres (Dt) was found. Stimulated S-phase cell nuclei were identified by incorporation of bromodeoxyuridine and simultaneous fluorescence labeling by anti-BrdU antibodies. In this case only the centromeres were FISH labeled. Of 301 cell nuclei about 187 (62%) were stimulated and among them 77 (41%) were paired according to the above mentioned criterion (Dc<0,15 dn). These results indicate that proliferating blood lymphocytes show a considerable tendency to centromere pairing. Assuming that the chromosome arm is probably localized between centromere and telomere with a homologous chromatin density, it may be concluded from the data that somatic pairing of whole chromosomes occurs preferentially during S-phase of the cell nucleus.

Paper Details

Date Published: 18 December 2000
PDF: 8 pages
Proc. SPIE 4164, Laser Microscopy, (18 December 2000); doi: 10.1117/12.410641
Show Author Affiliations
Shamci Monajembashi, Institute of Molecular Biotechnology (Germany)
Alexander Rapp, Institute of Molecular Biotechnology (Germany)
Michael Hausmann, Institute of Molecular Biotechnology (Germany)
Heike Dittmar, Institute of Molecular Biotechnology (Germany)
Karl-Otto Greulich, Institute of Molecular Biotechnology (Germany)


Published in SPIE Proceedings Vol. 4164:
Laser Microscopy
Karsten Koenig; Hans J. Tanke; Herbert Schneckenburger, Editor(s)

© SPIE. Terms of Use
Back to Top