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Proceedings Paper

Noninvasive tumor detection using spectrally-resolved in-vivo imaging
Author(s): Gennady Kostenich; Sol Kimel; Zvi Malik; Arie Orenstein
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Paper Abstract

A novel spectral image-analysis system was used for tumor fluorescence and reflectance imaging in an animal model and in patients. Transcutaneous fluorescence imaging was carried out on Balb/c mice bearing subcutaneous C26 colon carcinoma after intraperitoneal (i.p.) administration of 5-aminolevulinic acid (ALA), a metabolic precursor of protoporphyrin-IX (PP), and of a novel photosensitizer tetrahydroporphyrin (THP). Tumors were clearly observable by fluorescence detection using green light excitation. Tumor versus normal tissue uptake of the photosensitizing agents was determined by monitoring fluorescence intensity. Maximal PP accumulation in tumor was observed 3 h after i.p. injection of ALA, whereas THP showed selective accumulation in tumor 24 h after administration. Reflectance spectroscopy was employed to study pigmented human skin lesions (nevus, pigmented BCC and pigmented melanoma). In the near-infrared region (800-880 nm) pigmented BCC and melanoma exhibited a differently shaped reflectance spectrum compared to normal skin and nevus. Spatially and spectrally resolved imaging, in combination with mathematical algorithms (such as normalization, spectral similarity mapping and division) allowed unambiguous detection of malignancies. Optical biopsy results from a total of 51 patients showed 45 benign nevi, 3 pigmented BCC and 3 malignant melanomas, as confirmed by histology.

Paper Details

Date Published: 16 November 2000
PDF: 8 pages
Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); doi: 10.1117/12.409335
Show Author Affiliations
Gennady Kostenich, Sheba Medical Ctr. (Israel)
Sol Kimel, Sheba Medical Ctr. (Israel)
Zvi Malik, Bar-Ilan Univ. (Israel)
Arie Orenstein, Sheba Medical Ctr. (Israel)

Published in SPIE Proceedings Vol. 4161:
Optical Biopsy and Tissue Optics
Irving J. Bigio; Gerhard J. Mueller; Gerwin J. Puppels; Rudolf W. Steiner; Katarina Svanberg, Editor(s)

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