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Proceedings Paper

Endogenous fluorescence of normal and malignant fibroblast cultures
Author(s): Nili Grossman; Orna Chaims; Efrat Ilovitz; Shlomo Mark; Jacob Goppas; Ahmad Salman; Beny Cohen; Shaul Mordechai
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Paper Abstract

Light-induced Fluorescence (LIF) technique is based on fluorescence emitted from intracellular chromophores upon illumination of cells by monochromatic light. We compared LIF emitted from a pair of normal and malignant murine cell lines, differing in H-ras expression. The malignant cells fluoresced significantly less than the normal cells, upon excitation at 290 +/- 10 nm. For both cell types, fluorescence decreased with decreasing cell concentration, but at each concentration, the normal cells fluoresced more than the malignant cells. The effect of viability and metabolic stage of the cells on this pattern was compared. The difference among the cells was not due to a difference in protein or DNA content. Thus, this model system demonstrates the specific contribution of H-ras to sub-cellular chromophores, resulting in a significant difference in their autofluorescence intensity, while measuring both emission and excitation scans. This study suggests a potential use of the LIF technique to distinguish between normal and malignant cells and tissues.

Paper Details

Date Published: 16 November 2000
PDF: 9 pages
Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); doi: 10.1117/12.409326
Show Author Affiliations
Nili Grossman, Soroka Univ. Medical Ctr. and Ben-Gurion Univ. of the Negev (Israel)
Orna Chaims, Ben-Gurion Univ. of the Negev (Israel)
Efrat Ilovitz, Ben-Gurion Univ. of the Negev (Israel)
Shlomo Mark, Ben-Gurion Univ. of the Negev (Israel)
Jacob Goppas, Soroka Univ. Medical Ctr. (Israel)
Ahmad Salman, Ben-Gurion Univ. of the Negev (Israel)
Beny Cohen, Ben-Gurion Univ. of the Negev (Israel)
Shaul Mordechai, Ben-Gurion Univ. of the Negev (Israel)


Published in SPIE Proceedings Vol. 4161:
Optical Biopsy and Tissue Optics
Irving J. Bigio; Gerhard J. Mueller; Gerwin J. Puppels; Rudolf W. Steiner; Katarina Svanberg, Editor(s)

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