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Proceedings Paper

Raman spectroscopic studies on bacteria
Author(s): Kees Maquelin; Lin-P'ing Choo-Smith; Hubert Ph. Endtz; Hajo A. Bruining; Gerwin J. Puppels
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Paper Abstract

Routine clinical microbiological identification of pathogenic micro-organisms is largely based on nutritional and biochemical tests. Laboratory results can be presented to a clinician after 2 - 3 days for most clinically relevant micro- organisms. Most of this time is required to obtain pure cultures and enough biomass for the tests to be performed. In the case of severely ill patients, this unavoidable time delay associated with such identification procedures can be fatal. A novel identification method based on confocal Raman microspectroscopy will be presented. With this method it is possible to obtain Raman spectra directly from microbial microcolonies on the solid culture medium, which have developed after only 6 hours of culturing for most commonly encountered organisms. Not only does this technique enable rapid (same day) identifications, but also preserves the sample allowing it to be double-checked with traditional tests. This, combined with the speed and minimal sample handling indicate that confocal Raman microspectroscopy has much potential as a powerful new tool in clinical diagnostic microbiology.

Paper Details

Date Published: 16 November 2000
PDF: 7 pages
Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); doi: 10.1117/12.409323
Show Author Affiliations
Kees Maquelin, Erasmus Univ./Rotterdam and Univ. Hospital Rotterdam (Netherlands)
Lin-P'ing Choo-Smith, Erasmus Univ./Rotterdam and Univ. Hospital Rotterdam (Canada)
Hubert Ph. Endtz, Univ. Hospital Rotterdam (Netherlands)
Hajo A. Bruining, Erasmus Univ./Rotterdam and Univ. Hospital Rotterdam (Netherlands)
Gerwin J. Puppels, Erasmus Univ./Rotterdam and Univ. Hospital Rotterdam (Netherlands)

Published in SPIE Proceedings Vol. 4161:
Optical Biopsy and Tissue Optics
Irving J. Bigio; Gerhard J. Mueller; Gerwin J. Puppels; Rudolf W. Steiner; Katarina Svanberg M.D., Editor(s)

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