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Proceedings Paper

Raman spectroscopy for the diagnosis of dysplasia in columnar and squamous epithelium
Author(s): Catherine A. Kendall; Tom C. Bakker Schut; Nicholas Stone; Pela Stravroulaki; Gerwin J. Puppels; Hugh Barr M.D.
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Paper Abstract

The incidence of oesophageal and laryngeal cancer has risen over past decades. The early detection of disease is vital for improved prognosis. The current gold standard method of tissue diagnosis is histopathology, which is invasive, costly and somewhat subjective. Raman spectroscopy however has potential for the non-invasive, early, in-vivo diagnosis of the biochemical changes associated with malignancy. Good quality Raman spectra have been measured in vitro using oesophageal and laryngeal tissue. Multivariate analysis has been implemented using principal component and linear discriminant analysis techniques. Sensitivity and specificity of more than 80% has been achieved for the discrimination of dysplasia and cancer, for both oesophageal and laryngeal tissue. On comparison with histopathology these results are seen to be an improvement, since pathology lacks sensitivity and specificity due to the subjective nature of the diagnosis. Thus illustrating that excellent discrimination between normal, dyspalstic and cancerous tissue can be achieved using Raman Spectroscopy.

Paper Details

Date Published: 16 November 2000
PDF: 7 pages
Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); doi: 10.1117/12.409321
Show Author Affiliations
Catherine A. Kendall, Gloucestershire Royal Hospital (United Kingdom)
Tom C. Bakker Schut, Univ. Hospital Rotterdam (Netherlands)
Nicholas Stone, Gloucestershire Royal Hospital (United Kingdom)
Pela Stravroulaki, Southmead Hospital (United Kingdom)
Gerwin J. Puppels, Univ. Hospital Rotterdam (Netherlands)
Hugh Barr M.D., Gloucestershire Royal Hospital (United Kingdom)

Published in SPIE Proceedings Vol. 4161:
Optical Biopsy and Tissue Optics
Irving J. Bigio; Gerhard J. Mueller; Gerwin J. Puppels; Rudolf W. Steiner; Katarina Svanberg M.D., Editor(s)

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