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Proceedings Paper

Kinetics of photo processes in dye molecules and the resolution ofa two-photon excitation fluorescence microscope with nanosecond laser
Author(s): Evgeny Kleymionov; Valery S. Ivanov; Niko Meltola; Pekka E. Hanninen; Erkki J. Soini
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Paper Abstract

The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.

Paper Details

Date Published: 27 November 2000
PDF: 7 pages
Proc. SPIE 4316, International Conference on Lasers for Measurements and Information Transfer, (27 November 2000); doi: 10.1117/12.407664
Show Author Affiliations
Evgeny Kleymionov, St. Petersburg State Univ. (Russia)
Valery S. Ivanov, St. Petersburg State Univ. (Russia)
Niko Meltola, Univ. of Turku (Finland)
Pekka E. Hanninen, Univ. of Turku (Finland)
Erkki J. Soini, Univ. of Turku (Finland)


Published in SPIE Proceedings Vol. 4316:
International Conference on Lasers for Measurements and Information Transfer
Vadim E. Privalov, Editor(s)

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