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Proceedings Paper

Characterization of biofilms with a fiber optic spectrometer
Author(s): S. Krautwald; A. Tonyali; B. Fellerhoff; Hilmar Franke; A. Tamachkiarov; T. Griebe; H. C. Flemming
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Paper Abstract

Optical sensing is one promising approach to monitor bioflims in an early stage. Generally, natural bioflims are quite inhomogeneous, therefore we start the investigation with suspensions of dead bacteria in water as a simple model for a bioflim. An experimental arrangement based on a white light fiber optic spectrometer is used for measuring the density of a thin film with a local resolution in the order of several tim. The method is applied with model biofilms. In a computer controlled procedure reflectance spectra may be recorded at different positions in the x-y plane. Scanning through thin suspension regions of bacteria between glass plates allows an estimation of the refractive index of bacteria. Taking advantage of the light collecting property of the glass substrate a simple measurement of the fluorescence with local resolution is demonstrated as well.

Paper Details

Date Published: 15 December 2000
PDF: 10 pages
Proc. SPIE 4087, Applications of Photonic Technology 4, (15 December 2000); doi: 10.1117/12.406344
Show Author Affiliations
S. Krautwald, Univ. Duisburg (Germany)
A. Tonyali, Univ. Duisburg (Germany)
B. Fellerhoff, Univ. Duisburg (Germany)
Hilmar Franke, Univ. Duisburg (Germany)
A. Tamachkiarov, Univ. Duisburg (Germany)
T. Griebe, Univ. Duisburg (Germany)
H. C. Flemming, Univ. Duisburg (Germany)

Published in SPIE Proceedings Vol. 4087:
Applications of Photonic Technology 4
Roger A. Lessard; George A. Lampropoulos, Editor(s)

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