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Proceedings Paper

UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: an electron paramagnetic resonance (EPR) study
Author(s): Rachel Lubart; R. Lavie; Harry Friedmann; M. Sinyakov; Asher Shainberg; Haim Breitbart; Nili Grossman
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Paper Abstract

The stimulatory effect of low energy light (LEL) has been attributed to irradiation-induced ROS formation. In the present study we demonstrate that irradiating various cell cultures such as fibroblasts, cardiac and sperm cells with UVA or various light sources in the visible range results in singlet oxygen and OH radical formation. These radicals were monitored by using the EPR technique. We believe that the light induced ROS could mediate previously documented effects of LEL on these cells.

Paper Details

Date Published: 3 November 2000
PDF: 7 pages
Proc. SPIE 4159, Effects of Low-Power Light on Biological Systems V, (3 November 2000); doi: 10.1117/12.405915
Show Author Affiliations
Rachel Lubart, Bar-Ilan Univ. (Israel)
R. Lavie, Bar-Ilan Univ. (Israel)
Harry Friedmann, Bar-Ilan Univ. (Israel)
M. Sinyakov, Bar-Ilan Univ. (Israel)
Asher Shainberg, Bar-Ilan Univ. (Israel)
Haim Breitbart, Bar-Ilan Univ. (Israel)
Nili Grossman, Soroka Univ. Medical Ctr. (Israel)

Published in SPIE Proceedings Vol. 4159:
Effects of Low-Power Light on Biological Systems V
Tiina I. Karu; Rachel Lubart, Editor(s)

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