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Proceedings Paper

Role of PKC isozymes in low-power light-stimulated proliferation of cultured skin cells
Author(s): Nili Grossman; Vered Kleitman; Julia Meller; Roland Kaufmann; Nermin Akgun; Angelika Ruck; Etta Livneh; Rachel Lubart
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Paper Abstract

Exposure of cultured skin cells to low power visible light leads to a transiently stimulated proliferation. Facilitation of this response requires the presence of active PKC, elevation of intracellular calcium, and involves reactive oxygen species. In the present study, the role of PKC(alpha) and PCK(eta) was examined using paired murine fibroblasts, differing in the level of these isozymes expression. The ability of the cells to respond to low power UVA light or HeNe laser by stimulated proliferation was correlated with an active state or overexpression of PKC(alpha) , but not PKC(eta) . A parallel response was obtained in cells that were loaded with A1PcS4 before photosensitization. Whenever this latter treatment caused a light-stimulated inhibition, it was accompanied by the intracellular calcium and photosensitizer dynamics typical of the effect of PDT on rate epithelial cells. Accordingly, added antioxidants that suppressed light-stimulated proliferation also suppressed this light-stimulated inhibition. The model systems employed in this study are the first to demonstrate the specific effect of PKC isozymes on light-stimulated proliferation, in relation to oxidative stress, and indicate their dual role in light-tissue interaction.

Paper Details

Date Published: 3 November 2000
PDF: 7 pages
Proc. SPIE 4159, Effects of Low-Power Light on Biological Systems V, (3 November 2000); doi: 10.1117/12.405908
Show Author Affiliations
Nili Grossman, Ben Gurion Univ. of the Negev (Israel) and Soroka Univ. Medical Ctr. (Israel)
Vered Kleitman, Ben Gurion Univ. of the Negev (Israel)
Julia Meller, Ben Gurion Univ. of the Negev (Israel)
Roland Kaufmann, Goethe Univ. (Germany)
Nermin Akgun, Univ. of Ulm (Germany)
Angelika Ruck, Univ. of Ulm (Germany)
Etta Livneh, Ben Gurion Univ. of the Negev (Israel)
Rachel Lubart, Bar-Ilan Univ. (Israel)

Published in SPIE Proceedings Vol. 4159:
Effects of Low-Power Light on Biological Systems V
Tiina I. Karu; Rachel Lubart, Editor(s)

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