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Proceedings Paper

Confocal scanning fluorescence detection system and its optimization
Author(s): Rong Yang; Guoliang Huang; Jing Cheng; Minxian Wu; Guofan Jin
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Paper Abstract

Fluorescence detection by confocal scanning systems has advantages of large area detection, small focus depth, large numerical aperture, complete laser rejection, low background noise, high sensitivity and S/N ratio. It is a sensitive and precise method to record and analyze results of biological or biomedical assay, such as DNA hybridization assay or micro capillary electrophoresis assay on biochip. Parameters like power of laser or sensitivity of PMT are responsible for signal to noise ration and sensitivity of the system, which should be optimized relative to different situations. The structural design and experimental analysis on parameter optimization of a confocal scanning fluorescence detection system are presented in this paper.

Paper Details

Date Published: 11 October 2000
PDF: 4 pages
Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); doi: 10.1117/12.403993
Show Author Affiliations
Rong Yang, Tsinghua Univ. (China)
Guoliang Huang, Tsinghua Univ. (China)
Jing Cheng, Tsinghua Univ. (China)
Minxian Wu, Tsinghua Univ. (China)
Guofan Jin, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 4224:
Biomedical Photonics and Optoelectronic Imaging
Hong Liu; Qingming Luo, Editor(s)

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