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Proceedings Paper

Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer
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Paper Abstract

Green fluorescent protein based fluorescence resonance energy transfer is widely used in studies of inter-and intra-molecular dynamic interactions in alive cells. But it is a trade off between quantitative measurement and real time measurement because of the emission cross talk. In stead of using more than one excitation wavelength and several sets of filters, we proposed a more efficient correction scheme which needs only a single excitation and one filter set. This method makes use of the known emission spectra of the fluorophores and can be run in real time. The validity and advantages were demonstrated by experiments with chamaeleons.

Paper Details

Date Published: 11 October 2000
PDF: 5 pages
Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); doi: 10.1117/12.403990
Show Author Affiliations
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 4224:
Biomedical Photonics and Optoelectronic Imaging
Hong Liu; Qingming Luo, Editor(s)

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