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Proceedings Paper

Two-photon fluorescence imaging of rat aorta
Author(s): Xingsun Lin; Fei Sun; Hui Ma; Jingbo Zhao; Lei Jin; Die Yan Chen
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Paper Abstract

Two-photon excitation fluorescence microscopy has become increasingly popular in 3D fluorescence imaging of tissue samples. Observation depth is an important indicator for the performance of the technique. Using the principle of tissue optics, we analyzed quantitatively the effects of optical properties, such as absorption coefficient, scattering coefficient, and the scattering anisotropy factor of the tissue, to the observation depth. An analytical relation between the fluorescence and the observation depth has been derived and tested against experimental results for rat aorta elastic lamina.

Paper Details

Date Published: 11 October 2000
PDF: 6 pages
Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); doi: 10.1117/12.403959
Show Author Affiliations
Xingsun Lin, Tsinghua Univ. (China)
Fei Sun, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)
Jingbo Zhao, China-Japan Friendship Hospital (China)
Lei Jin, Tsinghua Univ. (China)
Die Yan Chen, Tsinghua Univ. (China)


Published in SPIE Proceedings Vol. 4224:
Biomedical Photonics and Optoelectronic Imaging
Hong Liu; Qingming Luo, Editor(s)

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