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Proceedings Paper

Formation of sequence-specific telomeric DNA loops via direct effects of psoralen-photosensitization on telomeres
Author(s): En-Hua Cao; Ai Chen; Xueguang Sun; Xiaoyan Zhang; Jingfen Qin; Dage Liu; Chen Wang; Chunli Bai
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Paper Abstract

DNA from HeLa cells was cleaved with Rasl and Hinf, and then purified by Bio-Gel P-2 column. Results showed that upon near-UV-irradiation, psoralen could be targeted to the repetitive sequences of telomeres. Large duplex loops with a tail were observed directly by AFM. The loop-tail junction position was assumed to be a tri- or tetra- strand DNA structure according to its apparent height, corresponding structure model was proposed. The psoralen cross-linking products increased with increase of irradiation time. The expression of p53 oncogene was significantly increased.

Paper Details

Date Published: 11 October 2000
PDF: 5 pages
Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); doi: 10.1117/12.403922
Show Author Affiliations
En-Hua Cao, Institute of Biophysics (China)
Ai Chen, Institute of Biophysics (China)
Xueguang Sun, Institute of Biophysics (China)
Xiaoyan Zhang, Institute of Biophysics (China)
Jingfen Qin, Institute of Biophysics (China)
Dage Liu, Institute of Chemistry (China)
Chen Wang, Institute of Chemistry (China)
Chunli Bai, Institute of Chemistry (China)


Published in SPIE Proceedings Vol. 4224:
Biomedical Photonics and Optoelectronic Imaging

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