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Proceedings Paper

Water structure and water/protein interactions in biological materials characterized by Raman spectroscopy
Author(s): Ole Faurskov Nielsen; Carina Koch Johansson; Kirsten L. Jakobsen; Daniel H. Christensen; Mette R. Wiegell; Thorvald Pedersen; Monika Gniadecka; Hans Christian Wulf; Peter Westh
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Paper Abstract

The R(v)-representation of the low-frequency Raman spectrum was used to investigate the low-frequency Raman spectrum of water. The advantages of using reduced representations in low-frequency Raman studies to display water structure are discussed. Tetrahedrically hydrogen bonded water molecules showed a characteristic low-frequency band with a peak maximum around 180 cm-1. O-18 and O-17 isotopic substitution revealed that the corresponding vibrational mode mainly involves displacements of the oxygen atoms, but no significant hydrogen motion. This mode can be used to monitor the existence of water with a bulk-like structure in biological macromolecular materials. To test its applicability NIR-FT-Raman spectroscopy was used in studies of biopolymers in order to avoid fluorescence.

Paper Details

Date Published: 29 September 2000
PDF: 9 pages
Proc. SPIE 4098, Optical Devices and Diagnostics in Materials Science, (29 September 2000); doi: 10.1117/12.401624
Show Author Affiliations
Ole Faurskov Nielsen, Univ. of Copenhagen (Denmark)
Carina Koch Johansson, Univ. of Copenhagen and Bispebjerg Hospital (Denmark)
Kirsten L. Jakobsen, Univ. of Copenhagen (Denmark)
Daniel H. Christensen, Univ. of Copenhagen (Denmark)
Mette R. Wiegell, Univ. of Copenhagen (United States)
Thorvald Pedersen, Univ. of Copenhagen (Denmark)
Monika Gniadecka, Bispebjerg Hospital (Denmark)
Hans Christian Wulf, Bispebjerg Hospital (Denmark)
Peter Westh, Roskilde Univ. (Denmark)


Published in SPIE Proceedings Vol. 4098:
Optical Devices and Diagnostics in Materials Science
David L. Andrews; David L. Andrews; Toshimitsu Asakura; Suganda Jutamulia; Wiley P. Kirk; Max G. Lagally; Ravindra B. Lal; James D. Trolinger, Editor(s)

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