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Proceedings Paper

High-numerical-aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy
Author(s): Yoshihiro Kawano; Chikara Abe; Teruo Kaneda; Yasushi Aono; Katsuyuki Abe; Keisuke Tamura; Susumu Terakawa
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Paper Abstract

We designed Apo 100X NA 1.65 and Plan Apo 60X NA 1.45 objective lenses. When we use three lenses for water- immersed specimens, the marginal angle available for the total internal reflection becomes larger compared with NA 1.40 objective lens. Therefore, the alignment of the laser beam to the objective lens becomes very easy. We also designed the TIRFM illuminator. A single mode fiber is connected between the laser and illuminator. The laser beam is conducted through the fiber and supplied to the illuminator with a pre-adjusted positioning. Almost no additional alignment of illumination light is necessary.

Paper Details

Date Published: 29 September 2000
PDF: 10 pages
Proc. SPIE 4098, Optical Devices and Diagnostics in Materials Science, (29 September 2000); doi: 10.1117/12.401623
Show Author Affiliations
Yoshihiro Kawano, Olympus America, Inc. (Japan)
Chikara Abe, Olympus America, Inc. (United States)
Teruo Kaneda, Olympus Optical Co., Ltd. (Japan)
Yasushi Aono, Olympus Optical Co., Ltd. (Japan)
Katsuyuki Abe, Olympus Optical Co., Ltd. (Japan)
Keisuke Tamura, Olympus Optical Co., Ltd. (Japan)
Susumu Terakawa, Hamamatsu Univ. School of Medicine (Japan)

Published in SPIE Proceedings Vol. 4098:
Optical Devices and Diagnostics in Materials Science
David L. Andrews; David L. Andrews; Toshimitsu Asakura; Suganda Jutamulia; Wiley P. Kirk; Max G. Lagally; Ravindra B. Lal; James D. Trolinger, Editor(s)

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