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Proceedings Paper

Cell-free protein synthesis in PDMS-glass hybrid microreactor
Author(s): Takatoki Yamamoto; Teruo Fujii; Takahiko Nojima; Jong Wook Hong; Isao Endo
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Paper Abstract

A living cell has numerous kinds of proteins while only thousands of that have been identified as of now. In order to discover and produce various proteins that are applicable to biotechnological, pharmaceutical and medical applications, cell-free protein synthesis is one of the most useful and promising techniques. In this study, we developed an inexpensive microreactor with temperature control capability for protein synthesis. The microreactor consists of a sandwich of glass-based chip and PDMS(polydimethylsiloxane) chip. The thermo control system, which is composed of a heater and a temperature sensor, is fabricated with an ITO (Indium Tin Oxide) resistive material on a glass substrate by ordinary microfabrication method based on photolithography and etching techniques. The reactor chamber and flow channels are fabricated by injection micromolding of PDMS. Since one can use thermo control system on a glass substrate repeatedly by replacing only the easily-fabricated and low-cost PDMS reactor chamber, this microreactor is quite cost effective. As a demonstration, a DNA template of a GFP (Green Fluorescent Protein) is transcribed and translated using cell-free extract prepared from Escherichia coli. As a result, GFP was successfully synthesized in the present microreactor.

Paper Details

Date Published: 18 August 2000
PDF: 8 pages
Proc. SPIE 4177, Microfluidic Devices and Systems III, (18 August 2000); doi: 10.1117/12.395674
Show Author Affiliations
Takatoki Yamamoto, Univ. of Tokyo (Japan)
Teruo Fujii, Univ. of Tokyo (Japan)
Takahiko Nojima, Waseda Univ. (Japan)
Jong Wook Hong, Univ. of Tokyo (United States)
Isao Endo, RIKEN-The Institute of Physical and Chemical Research (Japan)


Published in SPIE Proceedings Vol. 4177:
Microfluidic Devices and Systems III
Carlos H. Mastrangelo; Holger Becker, Editor(s)

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