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Proceedings Paper

Microfabricated plastic chips by hot embossing methods and their applications for DNA separation and detection
Author(s): Gwo-Bin Lee; Shu-Hui Chen; Guan-Ruey Huang; Yen-Heng Lin; Wang-Chou Sung
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Paper Abstract

Design and fabrication of microfluidic devices on polymethylmethacrylate (PMMA) substrates using novel microfabrication methods are described. The image of microfluidic devices is transferred from quartz master templates possessing inverse image of the devices to plastic plates by using hot embossing method. The micro channels on master templates are formed by the combination of metal etch mask and wet chemical etching. The micromachined quartz templates can be used repeatedly to fabricate cheap and disposable plastic devices. The reproducibility of the hot embossing method is evaluated after using 10 channels on different plastics. The relative standard deviation of the plastic channel profile from ones on quartz templates is less than 1%. In this study, the PMMA chips have been demonstrated as a micro capillary electrophoresis ((mu) -CE) device for DNA separation and detection. The capability of the fabricated chip for electrophoretic injection and separation is characterized via the analysis of DNA fragments (phi) X174. Results indicate that all of the 11 DNA fragments of the size marker could be identified in less than 3 minutes with relative standard deviations less than 0.4% and 8% for migration time and peak area, respectively. Moreover, with the use of near IR dye, fluorescence signals of the higher molecular weight fragments ($GTR 603 bp in length) could be detected at total DNA concentrations as low as 0.1 (mu) g/mL. In addition to DNA fragments (phi) X174, DNA sizing of hepatitis C viral (HCV) amplicon is also achieved using microchip electrophoresis fabricated on PMMA substrate.

Paper Details

Date Published: 18 August 2000
PDF: 10 pages
Proc. SPIE 4177, Microfluidic Devices and Systems III, (18 August 2000); doi: 10.1117/12.395648
Show Author Affiliations
Gwo-Bin Lee, National Cheng-Kung Univ. (Taiwan)
Shu-Hui Chen, National Cheng-Kung Univ. (Taiwan)
Guan-Ruey Huang, National Cheng-Kung Univ. (Taiwan)
Yen-Heng Lin, National Cheng-Kung Univ. (Taiwan)
Wang-Chou Sung, National Cheng-Kung Univ. (Taiwan)


Published in SPIE Proceedings Vol. 4177:
Microfluidic Devices and Systems III
Carlos H. Mastrangelo; Holger Becker, Editor(s)

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