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Proceedings Paper

Second-harmonic generation microscopy of tooth
Author(s): Fu-Jen Kao; Yung-Shun Wang; Mao-Kuo Huang; Sheng-Lung Huang; Ping Chin Cheng
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Paper Abstract

In this study, we have developed a high performance microscopic system to perform second-harmonic (SH)imaging on a tooth. The high sensitivity of the system allows an acquisition rate of 300 seconds/frame with a resolution at 512x512 pixels. The surface SH signal generated from the tooth is also carefully verified through micro-spectroscopy, polarization rotation, and wavelength tuning. In this way, we can ensure the authenticity of the signal. The enamel that encapsulates the dentine is known to possess highly ordered structures. The anisotrophy of the structure is revealed in the microscopic SH images of the tooth sample.

Paper Details

Date Published: 4 July 2000
PDF: 6 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390534
Show Author Affiliations
Fu-Jen Kao, National Sun Yat-sen Univ. (Taiwan)
Yung-Shun Wang, National Sun Yat-sen Univ. (Taiwan)
Mao-Kuo Huang, National Sun Yat-sen Univ. (Taiwan)
Sheng-Lung Huang, National Sun Yat-sen Univ. (Taiwan)
Ping Chin Cheng, SUNY/Buffalo (United States)

Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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