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Proceedings Paper

Applications of two-photon fluorescence microscopy in deep-tissue imaging
Author(s): Chen-Yuan Dong; Betty Yu; Lily Laiho Hsu; Peter D. Kaplan; D. Blankschstein; Robert Langer; Peter T. C. So
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Paper Abstract

Based on the non-linear excitation of fluorescence molecules, two-photon fluorescence microscopy has become a significant new tool for biological imaging. The point-like excitation characteristic of this technique enhances image quality by the virtual elimination of off-focal fluorescence. Furthermore, sample photodamage is greatly reduced because fluorescence excitation is limited to the focal region. For deep tissue imaging, two-photon microscopy has the additional benefit in the greatly improved imaging depth penetration. Since the near- infrared laser sources used in two-photon microscopy scatter less than their UV/glue-green counterparts, in-depth imaging of highly scattering specimen can be greatly improved. In this work, we will present data characterizing both the imaging characteristics (point-spread-functions) and tissue samples (skin) images using this novel technology. In particular, we will demonstrate how blind deconvolution can be used further improve two-photon image quality and how this technique can be used to study mechanisms of chemically-enhanced, transdermal drug delivery.

Paper Details

Date Published: 4 July 2000
PDF: 10 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390532
Show Author Affiliations
Chen-Yuan Dong, Massachusetts Institute of Technology (Taiwan)
Betty Yu, Massachusetts Institute of Technology (United States)
Lily Laiho Hsu, Massachusetts Institute of Technology (United States)
Peter D. Kaplan, Univerlever Edgewater Lab. (United States)
D. Blankschstein, Massachusetts Institute of Technology (United States)
Robert Langer, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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