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Proceedings Paper

Multiphoton microspectroscopy of biological specimens
Author(s): Bai-Ling Lin; Fu-Jen Kao; Ping Chin Cheng; Chi-Kuang Sun; RangWu Chen; YiMin Wang; JianCheng Chen; Yung-Shun Wang; Tzu-Ming Liu; Mao-Kuo Huang
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Paper Abstract

The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro- spectroscopy of biological specimen at high spatial resolution. Chloroplasts in mesophyll protoplast of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

Paper Details

Date Published: 4 July 2000
PDF: 5 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390530
Show Author Affiliations
Bai-Ling Lin, Institute of Molecular Biology (Taiwan)
Fu-Jen Kao, National Sun Yat-sen Univ. (Taiwan)
Ping Chin Cheng, SUNY/Buffalo (United States)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
RangWu Chen, Institute of Molecular Biology (Taiwan)
YiMin Wang, National Sun Yat-sen Univ. (Taiwan)
JianCheng Chen, National Sun Yat-sen Univ. (Taiwan)
Yung-Shun Wang, National Sun Yat-sen Univ. (Taiwan)
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)
Mao-Kuo Huang, National Sun Yat-sen Univ. (Taiwan)


Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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