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Proceedings Paper

Multiphoton fluorescence spectroscopy of flourescent bioprobes and biomolecules
Author(s): Ping Chin Cheng; Bai-Ling Lin; Fu-Jen Kao; Chi-Kuang Sun; Yung-Shun Wang; Tzu-Ming Liu; YiMin Wang; JianCheng Chen; Mao-Kuo Huang; Iain Johnson
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Paper Abstract

Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi- photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set- up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

Paper Details

Date Published: 4 July 2000
PDF: 5 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390527
Show Author Affiliations
Ping Chin Cheng, SUNY/Buffalo (United States)
Bai-Ling Lin, Institute of Molecular Biology (Taiwan)
Fu-Jen Kao, National Sun Yat-sen Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
Yung-Shun Wang, National Sun Yat-sen Univ. (Taiwan)
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)
YiMin Wang, National Sun Yat-sen Univ. (Taiwan)
JianCheng Chen, National Sun Yat-sen Univ. (Taiwan)
Mao-Kuo Huang, National Sun Yat-sen Univ. (Taiwan)
Iain Johnson, Molecular Probes Inc. (United States)

Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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