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Proceedings Paper

Photodynamic effect produced by HeNe radiation in Harderian glands of Wistar rats: an experimental model for PDT studies
Author(s): Edmyr Rosa dos Reis; Ester Maria Danielli Nicola; Konradin Metze; Jorge Humberto Nicola
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Paper Abstract

In rats, the Harderian Gland secret Protoporphirin IX which is retained at acinar lumina. Since this photosensitizer is important for PDT of malignant tumors, we propose to study this gland as a model to help understanding PDT with endogenous photosensitizers. Twenty Wistar SPF adult rats were submitted to surgical exposure of both Harderian glands, revealing red fluorescence upon UV, characterizing the protoporphirin IX presence. After that, one gland of each pair (one kept as control) was irradiated with an 8 mW HeNe (6328 angstrom) for 45 minutes, delivering about 2.7 joules/mm2. After 24 hours a group of 10 animals were scarified and the glands removed for histological analysis. The remaining animals were subjected to the same procedure but the glands were removed immediately after laser treatment. Histological and fluorescence analysis immediately after laser irradiation showed cell fragmentation with loss of acinar architecture with diffusion of protoporphirin in the cytoplasm of damaged cells, as well as interstitial edema. After 24 hours these alterations were more pronounced with accentuated loss of intraluminal protoporphirin and beginning of leukocytic demarcation of necrotic areas. The innate Harderian glands of rats, exposed to HeNe laser, showed a similar behavior as tumor tissue under PDT.

Paper Details

Date Published: 13 June 2000
PDF: 6 pages
Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); doi: 10.1117/12.388091
Show Author Affiliations
Edmyr Rosa dos Reis, Univ. Estadual de Campinas (Brazil)
Ester Maria Danielli Nicola, Univ. Estadual de Campinas (Brazil)
Konradin Metze, Univ. Estadual de Campinas (Brazil)
Jorge Humberto Nicola, Univ. Estadual de Campinas (Brazil)


Published in SPIE Proceedings Vol. 3914:
Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
Jeffrey O. Hollinger; Donald Dean Duncan; Jeffrey O. Hollinger; Donald Dean Duncan; Steven L. Jacques, Editor(s)

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