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Proceedings Paper

Photodynamic modulation of immune properties of blood cells
Author(s): Dmitry Lapotko; Tat'yana Romanovskaya; Vladimir P. Zharov
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Paper Abstract

The mechanisms of therapeutic action of laser radiation with and without photosensitizers were studied experimentally at cell level for living leukocytes. Single cell response to action of pathogens and to laser-based therapeutic processes was measured with photothermal (PT) image microscope. We have monitored cell functional properties during phagocytosis of bacteria S.aureus by human leukocytes. Laser therapy was applied in vitro to cell suspense and to the mixture of cells and microbes in three regimes: (1) low-power (30 - 50 mW/cm2) CW-laser radiation at 633 nm and at 670 nm; (2) photoactivated with 670 nm laser radiation Chlorin E6 at 0.2 mg/l (PDT); (3) photoactivated with 670 nm laser radiation aluminum disulfonated phthalocyanine (Photosens) at 0.2 mg/l (PDT). For all three regimes an increase of bactericidal effect was found only when the cells are involved into interaction with microbes. No bactericidal effect in the wide range of drug doses was found during direct application of PDT to bacteria in vitro. The best effect was found when the cells are treated with laser radiation only. Also laser radiation restored cell properties. As a result we suggest that immune stimulation depends more upon physical factors (laser) rather than upon pharmaceutical (photosensitizers).

Paper Details

Date Published: 13 June 2000
PDF: 10 pages
Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); doi: 10.1117/12.388076
Show Author Affiliations
Dmitry Lapotko, A.V. Luikov Heat and Mass Transfer Institute (United States)
Tat'yana Romanovskaya, International Sakharov Environmental Univ. (Belarus)
Vladimir P. Zharov, Bauman Moscow State Technical Univ. (United States)

Published in SPIE Proceedings Vol. 3914:
Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
Jeffrey O. Hollinger; Donald Dean Duncan; Donald Dean Duncan; Jeffrey O. Hollinger; Steven L. Jacques, Editor(s)

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