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Proceedings Paper

Light activation of the immune system part I: influence on G-class antibodies
Author(s): Vladimir P. Zharov; Vladimir S. Gevondyan; Natalya M. Gevondyan; Sergey A. Ermilov; Alla M. Volynskaya
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Paper Abstract

The experimental evidences for photochemical mechanism of light activation of immune system are presented. The experiments were made in vitro using serums from normal blood, from blood of people with secondary immunodeficiency containing low-avidity IgG (up to 75 - 90%), and pure IgG buffer solutions obtained from the same serums. LED arrays and halogen lamps with narrow spectral filters from UV to IR were used as optical monochromatic sources. We have discovered that light can activate immune system by IgG transformation from low-avidity state to high-avidity one. This change has multistage irreversible character and depends on light wavelength and intensity. The estimations of optical effects were made by determination of IgG functional activity and quaternary antibody structure through the number of accessible functional protein residues in the IgG buffer solutions. Both methods showed very correlated results. Each IgG avidity transformation stage correlated with definite change of antibody spatial structure that, to our opinion, corresponds to the phenomenon pass through the several intermediate metastable forms, which are maintained by different intermolecular bonds. The bioaction spectrum of discovered effects is also presented.

Paper Details

Date Published: 13 June 2000
PDF: 10 pages
Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); doi: 10.1117/12.388075
Show Author Affiliations
Vladimir P. Zharov, Bauman Moscow State Technical Univ. (United States)
Vladimir S. Gevondyan, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia)
Natalya M. Gevondyan, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia)
Sergey A. Ermilov, Bauman Moscow State Technical Univ. (United States)
Alla M. Volynskaya, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russia)


Published in SPIE Proceedings Vol. 3914:
Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
Jeffrey O. Hollinger; Donald Dean Duncan; Jeffrey O. Hollinger; Donald Dean Duncan; Steven L. Jacques, Editor(s)

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