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Proceedings Paper

Hydrodynamic study of the behavior of chondroitin sulphate under nondestructive laser irradiation of cartilage
Author(s): Emil N. Sobol; Alexander I. Omelchenko; Alexander P. Sviridov; Stephen E. Harding; Kornelia Jumel; Nicholas Jones
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Paper Abstract

The effects of laser irradiation on molecular mass and conformation of pure chondroitin sulphate dissolved in phosphate buffered saline were investigated using size exclusion chromatography/multi-angle light scattering (SEC/MALS) and sedimentation velocity in the analytical ultracentrifuge. In addition, cartilage pieces immersed in buffer were irradiated with a laser in order to study whether cartilage components may diffuse away from the matrix and into the surrounding aqueous medium as a result of laser treatment. Size exclusion chromatography/multi-angle light scattering and sedimentation velocity measurements showed that (a) laser irradiation decreases the molecular mass of chondroitin sulphate and (b) laser irradiation of cartilage induces diffusion of macromolecules into the medium. The results obtained allow us to understand the mechanism of stress relaxation and structural alterations in cartilage under non- destructive laser radiation.

Paper Details

Date Published: 13 June 2000
PDF: 6 pages
Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); doi: 10.1117/12.388025
Show Author Affiliations
Emil N. Sobol, Institute of Laser and Information Technologies (Russia)
Alexander I. Omelchenko, Institute of Laser and Information Technologies (Russia)
Alexander P. Sviridov, Institute of Laser and Information Technologies (Russia)
Stephen E. Harding, Univ. of Nottingham (United Kingdom)
Kornelia Jumel, Univ. of Nottingham (United Kingdom)
Nicholas Jones, Queen's Medical Ctr. (United Kingdom)


Published in SPIE Proceedings Vol. 3914:
Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
Jeffrey O. Hollinger; Donald Dean Duncan; Jeffrey O. Hollinger; Donald Dean Duncan; Steven L. Jacques, Editor(s)

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