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Proceedings Paper

3D reconstruction of a human heart fascicle using SurfDriver
Author(s): Robert J. Rader; Steven J. Phillips; Paul S. LaFollette Jr.
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Paper Abstract

The Temple University Medical School has a sequence of over 400 serial sections of adult normal ventricular human heart tissue, cut at 25 micrometer thickness. We used a Zeiss Ultraphot with a 4x planapo objective and a Pixera digital camera to make a series of 45 sequential montages to use in the 3D reconstruction of a fascicle (muscle bundle). We wrote custom software to merge 4 smaller image fields from each section into one composite image. We used SurfDriver software, developed by Scott Lozanoff of the University of Hawaii and David Moody of the University of Alberta, for registration, object boundary identification, and 3D surface reconstruction. We used an Epson Stylus Color 900 printer to get photo-quality prints. We describe the challenge and our solution to the following problems: image acquisition and digitization, image merge, alignment and registration, boundary identification, 3D surface reconstruction, 3D visualization and orientation, snapshot, and photo-quality prints.

Paper Details

Date Published: 6 June 2000
PDF: 9 pages
Proc. SPIE 3979, Medical Imaging 2000: Image Processing, (6 June 2000); doi: 10.1117/12.387721
Show Author Affiliations
Robert J. Rader, Temple Univ. (USA) and Temple Univ. Medical School (United States)
Steven J. Phillips, Temple Univ. (USA) and Temple Univ. Medical School (United States)
Paul S. LaFollette Jr., Temple Univ. (USA) and Temple Univ. Medical School (United States)

Published in SPIE Proceedings Vol. 3979:
Medical Imaging 2000: Image Processing
Kenneth M. Hanson, Editor(s)

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