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Proceedings Paper

Corneal equivalents: a replacement model for in-vivo 1540-nm laser exposure studies
Author(s): David J. Fletcher; Thomas E. Johnson; Michael A. Mitchell; Thomas E. Eurell; Pedro J. Rico; William P. Roach
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Paper Abstract

We investigate the use of in-vitro corneal equivalents as a replacement model for in-vivo rabbit corneas used in laser damage threshold studies. In-vitro corneal equivalents (CE) were exposed to 0.8 millisecond (ms), 1540 nanometer (nm) single laser light pulses ranging from 124 J/cm2 to 58 J/cm2. After exposure, CE's were evaluated opthalmoscopcopically, imaged using confocal microscopy and examined histologically to investigate the mechanisms of laser induced damage. Preliminary results indicate that the 50% damage threshold for CE's, ED50, is approximately 70 J/cm2 with a 0.5 mm diameter spot size. Additional data points are required to determine a statistically significant ED50. Preliminary exposures of in-vivo rabbit corneas using the same laser parameters as the CE's are also reported. Comparisons between the in-vivo and in-vitro models are presented. Histopathological images from both models show remarkable similarities in the location and extent of damage throughout the full thickness of each tissue.

Paper Details

Date Published: 17 May 2000
PDF: 8 pages
Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); doi: 10.1117/12.386288
Show Author Affiliations
David J. Fletcher, Uniformed Services Univ. of the Health Sciences (United States)
Thomas E. Johnson, Uniformed Services Univ. of the Health Sciences (United States)
Michael A. Mitchell, Uniformed Services Univ. of the Health Sciences (United States)
Thomas E. Eurell, Univ. of Illinois/Urbana-Champaign (United States)
Pedro J. Rico, Uniformed Services Univ. of the Health Sciences (United States)
William P. Roach, Uniformed Services Univ. of the Health Sciences (United States)


Published in SPIE Proceedings Vol. 3907:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X
R. Rox Anderson; Kenton W. Gregory; Eugene A. Trowers; David S. Robinson; Kenneth Eugene Bartels; Lou Reinisch; Reza S. Malek; C. Gaelyn Garrett; Lloyd P. Tate; Hans-Dieter Reidenbach; Timothy A. Woodward; Kenneth Eugene Bartels; Lawrence S. Bass; George M. Peavy; C. Gaelyn Garrett; Kenton W. Gregory; Nikiforos Kollias; Harvey Lui; Reza S. Malek; George M. Peavy; Hans-Dieter Reidenbach; Lou Reinisch; David S. Robinson; Lloyd P. Tate; Eugene A. Trowers; Timothy A. Woodward, Editor(s)

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