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Proceedings Paper

Use of osmotically active agents to alter optical properties of tissue: influence on the detected fluorescence signal from a subsurface target
Author(s): Gracie Vargas; Kin Foong Chan; Sharon L. Thomsen; Ashley J. Welch
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Paper Abstract

In this study, we used a technique that alters the optical properties of tissue to study the effects of tissue scattering on the detected fluorescence from a target placed under the tissue sample. By using hyper-osmotic solutions of glycerol or dimethyl sulfoxide, scattering in tissue samples was reduced substantially. A fluorescent film was placed underneath in vitro and in vivo samples of hamster skin. Excitation of the fluorescent film was achieved through the tissue and the fluorescent signal from the film monitored over time as an osmotically active agent was added to the tissue. The film had an absorption peak at 542 nm and an emission peak at 585 nm. The reduction in tissue scattering caused by the optical clearing agents led to an increase in the detected fluorescent signal (up to double the original signal). The increase was greater for 14 M dimethyl sulfoxide than for 13 M glycerol, and greater for in vivo skin than for in vitro skin. The study serves as a demonstration of the benefits of optical tissue clearing by osmotically active agents to the detected signal in an optical application. Temporary reduction of scattering in tissue will be useful in therapeutic and diagnostic applications that can benefit from an increase in the penetration depth of light.

Paper Details

Date Published: 17 May 2000
PDF: 6 pages
Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); doi: 10.1117/12.386242
Show Author Affiliations
Gracie Vargas, Univ. of Texas/Austin (United States)
Kin Foong Chan, Univ. of Texas/Austin (United States)
Sharon L. Thomsen, Univ. of Texas/Austin (United States)
Ashley J. Welch, Univ. of Texas/Austin (United States)


Published in SPIE Proceedings Vol. 3907:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X
R. Rox Anderson; Kenton W. Gregory; Eugene A. Trowers; David S. Robinson; Kenneth Eugene Bartels; Lou Reinisch; Reza S. Malek; C. Gaelyn Garrett; Lloyd P. Tate; Hans-Dieter Reidenbach; Timothy A. Woodward; Kenneth Eugene Bartels; Lawrence S. Bass; George M. Peavy; C. Gaelyn Garrett; Kenton W. Gregory; Nikiforos Kollias; Harvey Lui; Reza S. Malek; George M. Peavy; Hans-Dieter Reidenbach; Lou Reinisch; David S. Robinson; Lloyd P. Tate; Eugene A. Trowers; Timothy A. Woodward, Editor(s)

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