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Proceedings Paper

IR spectroscopic investigation of the inhibition of the glycation process by acetylsalicylic acid
Author(s): Virginia Otero de Joshi; Herminia Gil; Silvia Contreras; William Velasquez; Narahari V. Joshi
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Paper Abstract

An IR spectroscopic study was carried out at room temperature for Human Serum albumin (HSA) glycated with fructose and glucose and inhibited with acetylsalicylic acid. The glycation process was carried out in our laboratory by a conventional method to confirm earlier reported observation of the effect of glycation on the intensity variation of the IR spectra, particularly, in the range 1500 cm-1 to 1700 cm-1 and around 3300 cm-1. IR spectra reveal that the effects of glycation of HSA by fructose are more intense than with glucose, which is the expected. Bovine serum albumin was also glycated using Glucose-6-phosphate disodium salt, and gamma-globulin was glycate with glucose, As expected, the glycation process was more intense with glucose-t-phosphate disodium salt. Acetyl salicylic acid was also used and its inhibitor effects could be observed in both cases, with glucose and with glucose-6-phosphate disodium salt even though, to a smaller extent with the latter. This is consistent with the earlier data and is explained on the basis of the attachment of macromolecules to (epsilon) -NH2 groups of lysines. The experimental results confirm that acetylsalicylic acid, indeed, acts as an inhibitor by acetylation of the (epsilon) -NG2 group where the sugars are supposed to be attached.

Paper Details

Date Published: 8 May 2000
PDF: 5 pages
Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); doi: 10.1117/12.384949
Show Author Affiliations
Virginia Otero de Joshi, Univ. de Los Andes (Venezuela)
Herminia Gil, Univ. de Los Andes (Venezuela)
Silvia Contreras, Univ. de Los Andes (Venezuela)
William Velasquez, Univ. de Los Andes (Venezuela)
Narahari V. Joshi, Univ. de Los Andes (Spain)


Published in SPIE Proceedings Vol. 3918:
Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques
Anita Mahadevan-Jansen; Gerwin J. Puppels, Editor(s)

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