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Proceedings Paper

Surface-enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes
Author(s): Gerald Steiner; Reiner Salzer; Wolfgang B. Fischer; Christian Kuhne
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Paper Abstract

Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPA). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced IR absorption provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

Paper Details

Date Published: 8 May 2000
PDF: 7 pages
Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); doi: 10.1117/12.384946
Show Author Affiliations
Gerald Steiner, Dresden Univ. of Technology (Germany)
Reiner Salzer, Dresden Univ. of Technology (Germany)
Wolfgang B. Fischer, Dresden Univ. of Technology (Germany)
Christian Kuhne, Dresden Univ. of Technology (Germany)


Published in SPIE Proceedings Vol. 3918:
Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques
Anita Mahadevan-Jansen; Gerwin J. Puppels, Editor(s)

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