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Proceedings Paper

Characterization of DNA isolated from normal and cancerous ovarian tissues by ultraviolet resonance Raman spectroscopy
Author(s): Xiaojie Zhao; Michael A. Vinson; Donald C. Malins; Thomas G. Spiro
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Paper Abstract

We report significant differences in UV resonance Raman (UVRR) spectra of DNA samples from normal and cancerous tissues. The four bases of DNA, adenosine, thymine, guanosine and cytidine, can be enhanced in UVRR spectra, and their intensities are very sensitive to base stacking and DNA H-bonding. 14 DNA samples from patients at different stages of ovarian cancer, 5 from normal, 2 from primary, 3 from metastasis primary and 4 from distant metastasis tumor tissues, were characterized by 257, 238, 229, 220 and 210 nm-excited UVRR spectra. Raman spectral difference between normal and tumor DNA could be readily detected.

Paper Details

Date Published: 8 May 2000
PDF: 7 pages
Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); doi: 10.1117/12.384935
Show Author Affiliations
Xiaojie Zhao, Princeton Univ. (United States)
Michael A. Vinson, Pacific Northwest Research Foundation (United States)
Donald C. Malins, Pacific Northwest Research Foundation (United States)
Thomas G. Spiro, Princeton Univ. (United States)

Published in SPIE Proceedings Vol. 3918:
Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques
Anita Mahadevan-Jansen; Gerwin J. Puppels, Editor(s)

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