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Proceedings Paper

Principle of synchronous luminescence (SL) technique for biomedical diagnostics
Author(s): Tuan Vo-Dinh
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Paper Abstract

In the present study, the principle of synchronous luminescence (SL) is described for use in biomedical diagnostics. The SL method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelength interval between them. This SF procedure simplifies the emission spectrum and provides for greater selectivity and is used to detect subtle differences in the fluorescence emission of the biochemical species of cells and tissues. The SL method can be used to analyze tissue in vivo or to investigate spectral differences in normal and neoplastic cells in vitro. SL scans of skin tissues illustrate the use of the method. For in vitro diagnostics, a difference between the flourescent spectra of the normal rat liver epithelial and hepatoma cell lines were detected using synchronous fluorescence. The potential use of SF as s screening tool for cancer diagnosis is discussed.

Paper Details

Date Published: 3 May 2000
PDF: 8 pages
Proc. SPIE 3911, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems II, (3 May 2000); doi: 10.1117/12.384929
Show Author Affiliations
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)


Published in SPIE Proceedings Vol. 3911:
Biomedical Diagnostic, Guidance, and Surgical-Assist Systems II
Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D., Editor(s)

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