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Proceedings Paper

Time-domain 2D fluorescence lifetime microscopic measurement
Author(s): Jun Le Qu; Hanben Niu; Ji Li; Xiaomei Kuo; Junlan Zhou
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Paper Abstract

In this paper a new technique of time-domain 2-D fluorescence lifetime microscopic measurement is presented. A fluorescence lifetime imaging microscope system based on a synchroscan streak camera is developed, and a data processing method of this technique is described. Reference sample is used to test the system. Experimental results show that, when magnification of the microscope is 100X, temporal resolution of the system is 2ps and spatial resolution is 8 micrometer. When a finer aperture mask and a micro-shift-mechanical system are used, spatial resolution of less than 1 micrometer can be obtained.

Paper Details

Date Published: 25 April 2000
PDF: 8 pages
Proc. SPIE 3977, Medical Imaging 2000: Physics of Medical Imaging, (25 April 2000); doi: 10.1117/12.384525
Show Author Affiliations
Jun Le Qu, Shen Zhen Univ. (China)
Hanben Niu, Shen Zhen Univ. (China)
Ji Li, Shen Zhen Univ. (China)
Xiaomei Kuo, Shen Zhen Univ. (China)
Junlan Zhou, Shen Zhen Univ. (China)

Published in SPIE Proceedings Vol. 3977:
Medical Imaging 2000: Physics of Medical Imaging
James T. Dobbins; John M. Boone, Editor(s)

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