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Proceedings Paper

Immunosensing of trinitrotoluene using sol-gel glasses
Author(s): Esther H. Lan; Bruce S. Dunn; Jeffrey I. Zink
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Paper Abstract

Research in recent years has demonstrated that biological molecules such as enzymes, immunoglobulins, and other proteins can be immobilized in sol-gel derived matrices and retain their biological function. Development of glasses with encapsulated biomolecules has opened the possibility of solid-state optical biosensors for the detection and measurement of desired analytes. In this work, we report the successful use of sol-gel silica glasses with encapsulated antibodies for the detection of trinitrotoluene (TNT). Results show that both competitive immunoassay and displacement immunoassay are feasible using antibody-doped glasses, and these materials can detect TNT at < 1 ppm. Moreover, the sol-gel immobilized antibodies retained the ability to discriminate between TNT and an analog, trinitrobenzene. Finally, enhanced stability was observed in the sol-gel immobilized as compared to surface immobilized antibodies.

Paper Details

Date Published: 2 May 2000
PDF: 7 pages
Proc. SPIE 3943, Sol-Gel Optics V, (2 May 2000); doi: 10.1117/12.384335
Show Author Affiliations
Esther H. Lan, Univ. of California/Los Angeles (United States)
Bruce S. Dunn, Univ. of California/Los Angeles (United States)
Jeffrey I. Zink, Univ. of California/Los Angeles (United States)


Published in SPIE Proceedings Vol. 3943:
Sol-Gel Optics V
Bruce S. Dunn; Edward J. A. Pope; Helmut K. Schmidt; Masayuki Yamane, Editor(s)

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