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Proceedings Paper

Rapid analysis of gene copy numbers in cancer by FISH and automated spot counting from stacked confocal images
Author(s): Artyom M. Grigoryan; Edward R. Dougherty; Lukas Bubendorf; Juha Kononen; Ollie Kallionienmi
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Paper Abstract

Fluorescent in situ hybridization (FISH) is an excellent method for detection of gene copy number alterations in cancer and other diseases. A limitation of the technology is the tedious, inaccurate and often highly subjective spot counting. For a number of reasons, automation of FISH spot, counting has not been accomplished. FISH signals are often at different focal planes, resulting in interfering out-of- focus light. This paper describes current progress towards automated FISH spot counting, with particular reference to the previous technical limitations.

Paper Details

Date Published: 26 April 2000
PDF: 12 pages
Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); doi: 10.1117/12.384245
Show Author Affiliations
Artyom M. Grigoryan, Texas A&M Univ. (United States)
Edward R. Dougherty, Texas A&M Univ. (United States)
Lukas Bubendorf, National Institutes of Health (United States)
Juha Kononen, National Institutes of Health (United States)
Ollie Kallionienmi, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 3924:
Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation
Darryl J. Bornhop; Kai Licha, Editor(s)

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