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Proceedings Paper

Design of fluorescence dye-conjugated peptides for the receptor-targeted optical imaging of tumors using solid-phase peptide libraries
Author(s): Kai Licha; Sarah Bhargava; Andreas Becker; Carsten Hessenius; Jens Schneider-Mergener; Rudolph Volkmer-Engert
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Paper Abstract

We describe the design and properties of cyanine dye-peptide conjugates synthesized on solid supports for screening assays. With this approach, we demonstrate the feasibility of including a diagnostic molecule into a spot synthesis of membrane-bound peptide libraries in order to permit screening of complete dye-peptide conjugates for their potential as fluorescent contrast agents in biomedical optical imaging. A cellulose support, which is modified with a linker permitting cleavage of the dye-peptide conjugates from the support, was prepared. The attachment of dyes to the peptides is exemplified with carboxy-substituted indotricarbocyanines, which can be covalently linked to the N-terminal amino group or a lysin in the course of the synthesis. Several dye-peptide conjugates were obtained by automated peptide synthesis on resins. Furthermore, model sequences consisting of up to 11 amino acids were synthesized on cellulose in sufficient amounts and purity, thus permitting direct testing of these compounds in cell- based assays and fluorescence microscopy. The dyes show only negligible alterations in their absorption and fluorescence properties when attached to low molecular weight peptides. In conclusion, membrane-bound peptide libraries provide a powerful tool to generate large diversities of dye-labeled peptides and to make these compounds available for biological screening assays.

Paper Details

Date Published: 26 April 2000
PDF: 6 pages
Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); doi: 10.1117/12.384237
Show Author Affiliations
Kai Licha, Freie Univ. Berlin (Germany)
Sarah Bhargava, Humboldt Univ. zu Berlin (Germany)
Andreas Becker, Freie Univ. Berlin (Germany)
Carsten Hessenius, Humboldt Univ. zu Berlin (Germany)
Jens Schneider-Mergener, Humboldt Univ. zu Berlin (Germany)
Rudolph Volkmer-Engert, Humboldt Univ. zu Berlin (Germany)

Published in SPIE Proceedings Vol. 3924:
Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation
Darryl J. Bornhop; Kai Licha, Editor(s)

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