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Proceedings Paper

Real-time Ca ion wave imaging in living rat cardiac muscle cells by a confocal multiphoton microscope with a microlens-pinhole array scanner
Author(s): Katsumasa Fujita; Tomoyuki Kaneko; Osamu Nakamura; Masahito Oyamada; Tetsuro Takamatsu; Satoshi Kawata
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Paper Abstract

A real-time confocal multiphoton fluorescence microscope was developed to observe Ca2+ dynamics in living rat- cardiac muscle cells. The real-time imaging was achieved by multifocus excitation of a specimen with a rotating microlens-array disk. A pinhole-array disk for confocal detection was introduced in the microscope to improve the spatial resolution and the contrast of fluorescence images. Ca2+ wave and Ca2+ transient in cultured rat- cardiac cells were successfully observed with the developed microscope.

Paper Details

Date Published: 27 April 2000
PDF: 8 pages
Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); doi: 10.1117/12.384225
Show Author Affiliations
Katsumasa Fujita, Osaka Univ. (Japan)
Tomoyuki Kaneko, Osaka Univ. (Japan)
Osamu Nakamura, Osaka Univ. (Japan)
Masahito Oyamada, Kyoto Prefectural Univ. of Medicine (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)
Satoshi Kawata, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 3921:
Optical Diagnostics of Living Cells III
Daniel L. Farkas; Daniel L. Farkas; Robert C. Leif, Editor(s)

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