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Proceedings Paper

External optical imaging of freely moving mice with green fluorescent protein-expressing metastatic tumors
Author(s): Meng Yang; Eugene Baranov; Hiroshi Shimada; A. R. Moossa; Robert M. Hoffman
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Paper Abstract

We report here a new approach to genetically engineering tumors to become fluorescence such that they can be imaged externally in freely-moving animals. We describe here external high-resolution real-time fluorescent optical imaging of metastatic tumors in live mice. Stable high-level green flourescent protein (GFP)-expressing human and rodent cell lines enable tumors and metastasis is formed from them to be externally imaged from freely-moving mice. Real-time tumor and metastatic growth were quantitated from whole-body real-time imaging in GFP-expressing melanoma and colon carcinoma models. This GFP optical imaging system is highly appropriate for high throughput in vivo drug screening.

Paper Details

Date Published: 27 April 2000
PDF: 4 pages
Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); doi: 10.1117/12.384220
Show Author Affiliations
Meng Yang, AntiCancer, Inc., Univ. of California/San Diego, and Yokohama City Univ. School of Medicin (United States)
Eugene Baranov, AntiCancer, Inc. (United States)
Hiroshi Shimada, Yokohama City Univ. School of Medicine (Japan)
A. R. Moossa, Univ. of California/San Diego (United States)
Robert M. Hoffman, AntiCancer, Inc. and Univ. of California/San Diego (United States)


Published in SPIE Proceedings Vol. 3921:
Optical Diagnostics of Living Cells III
Daniel L. Farkas; Daniel L. Farkas; Robert C. Leif, Editor(s)

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