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Proceedings Paper

Magnetic tweezers microscope for cellular manipulation
Author(s): Chen-Yuan Dong; Hayden Huang; Jason D. B. Sutin; Hyuk-Sang Kwon; George E. Cragg; R. Gilbert; Richard T. Lee; Enrico Gratton; Roger D. Kamm; Douglas A. Lauffenburger; Peter T. C. So
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Paper Abstract

We present the design of a magnetic tweezers microscope for cellular manipulation. Our design allows versatile and significant 3D stress application over a large sample region. For linear force application, forces up to 250 pN per 4.5 micrometers magnetic bead can be applied. Finite element analysis shows that variance in force level is around 10 percent within an area of 300 X 300 micrometers 2. Our eight-pole design potentially allows 3D liner force application and exertion of torsional stress. Furthermore, our design allows high resolution imaging using high numerical aperture objective. Both finite element analysis of magnetic field distribution and force calibration of our design are presented. As a feasibility study, we incubated fibronectin coated 4.5 micrometers polystyrene beads with Swiss 3T3 mouse fibroblast cells. Under application around 250 pN of force per magnetic particle, we observed relative movement between attached magnetic and polystyrene beads to be on the order of 1 micrometers . Elastic, viscoelastic, and creeping responses of cell surfaces were observed. Our results are consistent with previous observations using similar magnetic techniques.

Paper Details

Date Published: 27 April 2000
PDF: 10 pages
Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); doi: 10.1117/12.384210
Show Author Affiliations
Chen-Yuan Dong, Massachusetts Institute of Technology (Taiwan)
Hayden Huang, Massachusetts Institute of Technology (United States)
Jason D. B. Sutin, Univ. of Illinois/Urbana-Champaign (United States)
Hyuk-Sang Kwon, Massachusetts Institute of Technology (United States)
George E. Cragg, Massachusetts Institute of Technology (United States)
R. Gilbert, Massachusetts Institute of Technology (United States)
Richard T. Lee, Brigham and Womens Hospital (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)
Roger D. Kamm, Massachusetts Institute of Technology (United States)
Douglas A. Lauffenburger, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 3921:
Optical Diagnostics of Living Cells III
Daniel L. Farkas; Daniel L. Farkas; Robert C. Leif, Editor(s)

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